Description
Resorufin sodium salt is an anionic tricyclic dye with fluorescence. It can be used as an oxidation-reduction indicator in cell viability assays for both aerobic and anaerobic respiration.
Chemical Properties
Dark Purple Solid
Uses
Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis.
Uses
Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye.
General Description
Resorufin is a fluorescent indicator.
Purification Methods
The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.]
The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.]
The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.]
