Uses
3-Buten-1-amine is an aliphatic terminal amine used for organic synthesis and in proteomics research. Its hydrochloride salt is used to improve the detection of neurodegenerative alzheimers disease in ELISA titrations by reducing the background due to non-specific associations of molecules and proteins.
Synthesis
Hydrazine hydrate (20.0 g, 0.40 mol) was added to a solution of N-(3-buten-1-yl)phthalimide (40.2 g, 0.20 mol) in ethanol (200 mL). The reaction solution was heated to reflux for about 1.5 h. The reaction solution was cooled, 40 mL of concentrated hydrochloric acid was added dropwise and filtered after stirring for 10 min. The filtrate was adjusted to pH 7-8 with 5M potassium hydroxide aqueous solution, filtered, the filtrate was extracted with methyl tert-butyl ether (100mL*4), the organic phases were combined, dried with anhydrous potassium carbonate, filtered, and the solvent was removed by evaporation under reduced pressure to give 11.5 g of a white solid, i.e., 3-buten-1-amine.
