Description
DAF-
2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-
2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-
5 nM.
Uses
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.
Uses
Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.
References
[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI:
10.1248/cpb.46.373[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI:
10.1016/s0014-5793(98)00440-2
