DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM.
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.
Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.
