TRIS MALEATE

Chemical Properties

WHITE FINE CRYSTALLINE POWDER

Uses

Trizma^? maleate has been used:

• in the rehydration of sputum cytospin samples for immunocytochemical analysis
• in the incubation of spinal cords sections prior to nucleoside diphosphatase assay
• as a component of alkaline phosphate (AP) staining for embryonic stem cells

Uses

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma maleate is used in the preparation of Tris maleate buffers with lower effective pH buffering capacity than Tris buffers. Tris maleate buffers may be used in various grow media, in tissue preparations and as a diluent or washing buffer.

Definition

ChEBI: A maleate salt resulting from the reaction of tris and maleic acid in the molar ratio tris:maleic acid = 2:1.