Tris(hydroxymethyl)aminomethane acetate salt

Description

Tris(hydroxymethyl)aminomethane acetate salt(Tris-Acetate-EDTA) is a buffer solution commonly used in molecular biology and related fields.

Chemical Properties

White Crystalline Powder

Uses

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

Uses

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Definition

ChEBI: An acetate salt resulting from the reaction of equimolar amounts of tris and acetic acid.