D-Lysine homopolymer hydrobromide

Uses

Poly-D-lysine hydrobromide is used for the following applications:

• Used in animal cell culture
• Rat cortical neuron cultures
• Cell culture and siRNA treatment
• Primary cell cultures and adult neuronal network model
• Cortical astrocyte culture
• Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)
• NPC collection and culture conditions

Uses

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.