Chemical Properties
White to off-white powder
Uses
For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.
Uses
(+)-Biotin 4-nitrophenyl ester may be used in the preparation of biotin tagged ARC (adenosine-oligoarginine conjugate) derivative during cellular protein uptake studies. It may also be used to prepare biotinylated quinazoline derivatives, which shows inhibitory activities against VEGFR-2 (vascular endothelial growth factor receptor-2) tyrosine kinase. (+)-Biotin 4-nitrophenyl ester undergoes hydrolysis in the presence of Na[
17O]H to form [
17O
2]biotin, which can be used in solid-state
17O NMR spectroscopic experiments to analyze large protein-ligand complexes.
General Description
The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.
Biochem/physiol Actions
Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling. BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction. Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.
Purification Methods
The ester has been recrystallised by dissolving 2g in 95% EtOH (30mL), heated to dissolve, then cooled in an ice-water bath. The crystals are collected, washed with ice-cold 95% EtOH (5mL) and dried over P2O5. The RF on silica plates (CHCl3/MeOH 19:1) is 0.19 [Bodanszky & Fagan J Am Chem Soc 99 235 1977].
