7385-67-3
基本信息
尼罗红
NILE BLUE A OXAZONE
NILE RED
9-(diethylamino)-5h-benzo[a]phenoxazin-5-on
nile
9-(diethylamino)benzo[a]phenoxazin-5(5H)-one
FLUORESCENCE QY-STANDARD E CERTIFIED
Fluorescent Spectral Standard E, certified
9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on
Nile Red, pure, 99%
9-Diethylamino-benzo[a]phenoxazin-5-one
7-(Diethylamino)-1,2-[1,3]butadieno-3H-phenoxazine-3-one
9-Diethylamino-5H-benzo[a]phenoxazine-5-one
Oxazine O-17M
Phenoxazone
物理化学性质
| 熔点 | 203-205 °C(lit.) |
| 熔点 | 203-205 °C(lit.) |
| 沸点 | 457.53°C (rough estimate) |
| 密度 | 1.1871 (rough estimate) |
| 折射率 | 1.5700 (estimate) |
| 储存条件 | 2-8°C |
| 储存条件 | room temp |
| 溶解度 | 在甲醇中可溶1mg/mL |
| 酸度系数(pKa) | 4.08±0.20(Predicted) |
| 形态 | 粉末 |
| 颜色 | 深红色至栗色 |
| 外观 | Green to Dark Green Solid |
| 最大波长(λmax) | 553 nm |
| BRN | 279110 |
| 稳定性 | 稳定的。与强氧化剂不相容。 |
| InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| SMILES | C12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1 |
| CAS 数据库 | 7385-67-3(CAS DataBase Reference) |
| EPA化学物质信息 | 5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- (7385-67-3) |
安全数据
| 危险性符号(GHS) |  GHS07 |
| 警示词 | 警告 |
| 危险性描述 | H302-H315-H319-H335 |
| 防范说明 | P261-P280-P301+P312-P302+P352-P305+P351+P338 |
| 安全说明 | 24/25 |
| 安全说明 | S24/25 |
| WGK Germany | 3 |
| WGK Germany | 3 |
| F | 8-10 |
| TSCA | TSCA listed |
| 海关编码 | 29349990 |
应用领域
常见问题列表
2）染色步骤
① 尼罗红染色液配制：a.母液准备：取适量的尼罗红（Mw：318.37g/mol）用无水DMSO充分溶解制备1mM储存液，按照单次用量分装冻存，避免反复冻融，避光保存。b.工作液准备：用HHBS或生理缓冲液(pH 7.0)将母液按1:1000稀释为1×尼罗红工作液，漩涡混匀。
② 用检测化合物处理细胞一段时间；
③ 离心并调整细胞浓度为1-5×10^5cell/管；
④ 用500µL尼罗红工作液重悬细胞；
⑤ 室温或37ºC避光孵育5-10min；
⑥ 吸掉染色工作液，用HHBS或适当缓冲液清洗细胞；
⑦ 用500µL预热的HHBS或培养基重悬细胞，使细胞密度为1-5×105cell/管；
⑧ 荧光显微镜或流式细胞仪检测荧光信号，Ex/Em=552/636nm。
【注意】：① 对于贴壁细胞，可用HHBS或适当缓冲液清洗细胞，然后直接加入尼罗红工作液孵育；② 细胞也可预固定，然后用尼罗红工作液染色。
| Target | Value |
|
lipid droplet
() |
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.
知名试剂公司产品信息
Nile Red, pure, 99%(7385-67-3) Acros Organics
Nile Red(7385-67-3) TCI Shanghai
尼罗红价格(试剂级)
| 报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
| 2026/09/15 | HY-D0718 | 尼罗红 Nile Red | 7385-67-3 | 5 mg | 162.5元 |
| 2026/09/15 | HY-D0718 | 尼罗红 Nile Red | 7385-67-3 | 10mg | 260元 |
| 2026/09/15 | HY-D0718 | 尼罗红 Nile Red | 7385-67-3 | 50mg | 422.5元 |