108964-32-5
基本信息
AM荧光探针
FURA-2/AM荧光探针
钙离子荧光探针 Fura 2-AM
荧光钙探针 Fura 2-AM
1-[2-(5-CARBOXYOXAZOL-2-YL)-6-AMINOBENZOFURAN-5-OXYL]-2-(2'-AMINO-5'-METHYLPHENOXY)-ETHANE-N,N,N,N-TETRAACETIC ACID PENTAACETOXYMETHYL ESTER
ACETOXYMETHYL ESTER OF FURA 2
FURA 2/AM
FURA-2 PENTAKIS(ACETOXYMETHYL) ESTER
FURA 2/AM in Solution
fura 2-am yellow powder
Fura?-AM
FURA2/AM *1SET = 10x100UG**
FURA-2/AM, FOR FLUORESCENCE
1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)-ethane-N,N,N',N'-tetraaceticacidpentaacetoxymethylester
2-(6-(Bis(2-((acetyloxy)methoxy)-2-oxoethyl)amino)-5-(2-(2-(bis(2-((acetylxoy)methoxy)-2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl)-5-oxazolecarboxylic acid (acetyloxy)methyl ester
2-[6-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]benzofuran-2-yl]-5-oxazolecarboxylic acid acetyloxymethyl ester
Fura 2 acetoxymethyl ester
2-[6-[Bis(hydroxycarbonylmethyl)amino]-5-[2-[2-[bis(hydroxycarbonylmethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid acetoxymethyl ester
Fura-2 acetoxymethyl
物理化学性质
| 熔点 | >250℃ |
| 沸点 | 975.9±75.0 °C(Predicted) |
| 密度 | 1.400±0.06 g/cm3(Predicted) |
| 储存条件 | −20°C |
| 储存条件 | −20°C |
| 溶解度 | DMSO: soluble |
| 溶解度 | 可溶于DMSO |
| 酸度系数(pKa) | 1.89±0.50(Predicted) |
| 形态 | 粉末 |
| 颜色 | 黄色 |
| 敏感性 | 感光 |
| 最大波长(λmax) | 370nm |
| BRN | 8183748 |
| 生物领域应用 | Calcium indicator; monitoring membrane potential; treating ischemia |
| CAS 数据库 | 108964-32-5(CAS DataBase Reference) |
知名试剂公司产品信息
常见问题列表
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6. Mount the coverslip on the imaging chamber.