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• Preparation

  method for extracting WELAN GUM from WELAN GUM fermentation broth involves centrifuging and extracting the fermentation broth to obtain the WELAN GUM. The method includes the following steps: A. Fermentation Broth Pretreatment A1. At pH 6-8, add lysozyme with an activity of 100,000 u/g to the fermentation broth. The concentration of lysozyme in the fermentation broth is 100-200 ppm. After stirring evenly, maintain the reaction for 3-5 hours. A2. Heat the fermentation broth from step A1 to 130℃-140℃, maintain for 5-8 minutes, then lower to 60-70℃. Add acidic protease with an activity of 50,000 u/g. The concentration of acidic protease in the fermentation broth is 200-400 ppm. React at pH 3-4 and a temperature of 50℃-60℃ for 2-4 hours to obtain the pretreated fermentation broth.

  B. First Extraction: Mix the pretreated fermentation broth with an ethanol solution containing at least 85% (v/v) at a ratio of 1:0.5-0.6, and stir for 30-40 minutes. Adjust the pH of the mixture to 8.0-9.5 to allow the gel to precipitate as flocculent precipitate.

  C. First Centrifugation: Separate the solid and liquid components of the mixture from step B to obtain fibrous gel.

  D. Second Extraction: Mix the fibrous gel prepared in step C with an ethanol solution containing at least 85% (v/v) at a ratio of 1:0.3-0.5, and stir for 30-35 minutes. Adjust the pH of the mixture to 6.0-6.5 to allow the gel to precipitate as flocculent precipitate.

  E. Second Centrifugation: Separate the solid and liquid components of the mixture prepared in step D to obtain fibrous gel.

  F. Three-stage elution: Mix the fibrous gel prepared in step E with an ethanol solution containing at least 85% by volume in a ratio of 1:0.4-0.6, stir for 30-35 minutes, adjust the pH of the mixture to 6.0-6.5, causing the gel to precipitate as flocculent precipitate. Separate the mixture into solid and liquid phases to obtain the fibrous gel.

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