609-99-4

Name	3,5-Dinitrosalicylic acid
CAS	609-99-4
EINECS(EC#)	210-204-3
Molecular Formula	C7H4N2O7
MDL Number	MFCD00149501
Molecular Weight	228.12
MOL File	609-99-4.mol

Synonyms

2-HYDROXY-3,5-DINITROBENZOIC ACID 3,5-DINITRO-2-HYDROXYBENZOIC ACID 3,5-DINITRO-2-HYDROXYBENZOIC ACID HYDRATE 3,5-DINITROSALICYCLIC ACID 3,5-DINITROSALICYLIC ACID 3,5-DINITROSALICYLIC ACID HYDRATE LABOTEST-BB LT00060163 RARECHEM AL BE 0171 2-hydroxy-3,5-dinitro-benzoicaci 3,5-dinitrosalicylic 3,5-dinitro-salicylicaci Benzoic acid, 2-hydroxy-3,5-dinitro- Salicylic acid, 3,5-dinitro- 3,5-DinitrosalicyclicAcidGr 3-5,DinitroSalicylicAcidForSynthesis 3-5,DinitroSalicylicAcidForSynthesis-(3-5,Dinitro-2-HydroxybenzoicAcid) 3,5-DinitrosalicylicAcid99% 3,5-DinitrosalicyclicAcidForSynthesis 3-5,DinitroSalicylicAcidGr 3-5, DINTRO SALICYLIC ACID FOR SYNTHESIS

Chemical Properties

Appearance	solid
Melting point	168-172 °C (lit.)
Boiling point	369.91°C (rough estimate)
density	1.7914 (rough estimate)
refractive index	1.5300 (estimate)
storage temp.	room temp
solubility	H2O: soluble50mg/mL
form	Powder
pka	1.57±0.10(Predicted)
color	Yellow
PH Range	1.3-1.8 (10g/L @ 20°C)
Stability:	Stable. Incompatible with strong oxidizing agents, strong bases, strong acids.
Water Solubility	soluble
BRN	2220661
CAS DataBase Reference	609-99-4(CAS DataBase Reference)
NIST Chemistry Reference	3,5-Dinitrosalicylic acid(609-99-4)
EPA Substance Registry System	609-99-4(EPA Substance)

Safety Data

Hazard Codes	Xn,Xi
Risk Statements	R22:Harmful if swallowed. R37/38:Irritating to respiratory system and skin . R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S22:Do not breathe dust . S24/25:Avoid contact with skin and eyes . S36:Wear suitable protective clothing . S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . less more
RIDADR	2811
WGK Germany	3
RTECS	VO2965000
Hazard Note	Irritant
TSCA	Yes
HazardClass	6.1(b)
PackingGroup	III
HS Code	29182990

Hazard Information

Chemical Properties

solid

Uses

3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars.

Uses

3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars.

Uses

3,5-Dinitrosalicylic acid is used in the quantification of reducing sugars by colorimetry. It is utilized for the analysis of glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. It is also used to detect reducing substances in urine and for quantifying carbohydrate level in blood. It acts as an important raw material and an intermediate in the production of organic chemicals such as agrochemicals, organic synthesis, pharmaceuticals and dyes.

Definition

ChEBI: A monohydroxybenzoic acid consisting of 2-hydroxybenzoic acid having nitro substituents at the 3- and 5-positions. It is used in colorimetric testing for the presence of free carbonyl groups (C2O) in reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Purification Methods

Crystallise the acid from H2O. [Beilstein 10 IV 270.]

Material Safety Data Sheet(MSDS)

Spectrum Detail

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