ChemicalBook CAS DataBase List 2650-17-1

2650-17-1

Name	Xylene Cyanole FF
CAS	2650-17-1
EINECS(EC#)	224-728-5
Molecular Formula	C25H27N2NaO6S2
MDL Number	MFCD00040657
Molecular Weight	538.61
MOL File	2650-17-1.mol

Chemical Properties

Appearance	dark green or blue-grey crystals
Melting point	295°C
storage temp.	Store at RT.
solubility	H2O: complete1mg/mL, clear, blue
Colour Index	42135
form	Solid
color	Green
PH Range	2.9 (purple) - 4.6 (green)(Methyl Orange - Xylene Cyanol)
Stability:	Stable. Moisture-sensitive. Incompatible with strong oxidizing agents.
Water Solubility	Soluble in water.
CAS DataBase Reference	2650-17-1(CAS DataBase Reference)
EPA Substance Registry System	2650-17-1(EPA Substance)

Safety Data

Hazard Codes	Xi
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . S24/25:Avoid contact with skin and eyes . less more
WGK Germany	3
TSCA	Yes
HS Code	32041900

Hazard Information

Chemical Properties

dark green or blue-grey crystals

Uses

Suitable as a tracking dye for DNA sequencing.

Uses

Xylene Cyanol FF is a tracking dye commonly used for nucleic acids. The compound has been used in both electrophoresis polyacrylamide and agarose gels for various evaluations including DNA sequencing. The compound has also been used in a mobility shift analysis of ssDNA. In addition, two highly sensitive spectrophotometric assays have been developed for the detection of platinum (IV) (sc-250738, Platinum(IV) chloride) and Chromium (sc-214714) with the use of Xylene Cyanol FF. As an organic dye-inactivated Sendai virus vaccine, Xylene Cyanol FF displayed strong protection against the virus.

Preparation

4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product.

General Description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Properties and Applications

bright blue. Soluble in water to blue.

Standard	Light Fastness	Soaping		Persperation Fastness	Oxygen bleaching		Fastness to seawater
Standard	Light Fastness	Fading	Stain	Persperation Fastness	Fading	Stain	Fading	Stain
ISO	2	2	3-4	2-3	1		2-3	1
AATCC	2	4-5	4-5	2-3	1		2	2

Material Safety Data Sheet(MSDS)

Spectrum Detail

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