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BDP^®-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor to BDP-aminoacetaldehyde (BAAA), a cell-permeant fluorescent substrate for aldehyde dehydrogenase (ALDH). Inside the cell, BAAA is converted by intracellular ALDH to BDP-aminoacetate (BAA), which accumulates in cells and can serve as an indicator of high ALDH activity.

BAAA-DA can be used for identifying, isolating, and sorting cells with high levels of ALDH, such as human hematopoietic progenitor cells and cancer stem cells.

BAA has an excitation maximum of 503 nm and an emission maximum of 509 nm.

BODIPY aminoacetaldehyde diethyl acetal is a stable precursor to BODIPY-aminoacetaldehyde and can be converted into BODIPY-aminoacetaldehyde under acidic conditions. BODIPY-aminoacetaldehyde is a fluorescent substrate for aldehyde dehydrogenase (ALDH)[1].

BODIPY-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor to BODIPY-aminoacetaldehyde, a cell-permeable fluorescent substrate for aldehyde dehydrogenase (ALDH).1,2 BODIPY-aminoacetaldehyde diethyl acetal is converted under acidic conditions to BODIPY-aminoacetaldehyde (BAAA).2 BAAA is cell-permeant and is converted intracellularly by ALDH to BODIPY aminoacetate (BAA), which is retained by cells and can be used to identify cells with high ALDH activity.1 BAA is a substrate for the efflux pump P-glycoprotein (P-gp) but co-application of BAAA with a P-gp inhibitor, such as verapamil , inhibits BAA efflux.2 BAAA-DA has been used to isolate human hematopoietic progenitor cells, which have high ALDH activity, and via flow cytometry to sort cancer stem cells that contain high levels of ALDH.1,3 BAA used in cells can be excited at 488 nm and displays an emission maximum of 512 nm.4

1.Storms, R.W., Trujillo, A.P., Springer, J.B., et al.Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activityProceedings of the National Academy of Sciences of the United States of America96(16)9118-9123(1999) 2.Smith, C.A., Colvin, M., Storms, R.W., et al.BODIPY aminoacetaldehyde diethyl acetal08010501.81-15(2010) 3.Leng, Z., Yang, Z., Li, L., et al.A reliable method for the sorting and identification of ALDHhigh cancer stem cells by flow cytometryExp. Ther. Med.(2017) 4.Pomper, M.G., Wang, H., Minn, I., et al.Red fluorescent aldehyde dehydrogenase (ALDH) substrate(2015)

503/509 nm