2140-71-8

2''-O-Methylguanosine is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2’-O-methyltransferase, using S-adenosyl-L-methionine (Item Nos. 16376 | 13956) as a substrate. Through its interaction with other modified nucleosides, 2''-O-methylguanosine is thought to stabilize the structure of the tRNA. 2''-O-Methylguanosine can also be found in rRNA. Normal and modified nucleosides, including 2''-O-methylguanosine, have been shown to be secreted by a natural suppressor cell line and induce apoptosis in K562/Molt4 target cells.

White Solid

Guanosine analog.

ChEBI: Guanosine with the hydrogen on the hydroxyl at position C-2' substituted with a methyl group.

8-Thio-2'-O-methylguanosine (1 mmole) is dissolved in water and the solution is heated at reflux with Raney nickel for 6 h. The product is isolated by passing the solution through a short pad of celite and is further crystallizing to afford 2'-O-methylguanosine.

Store at -20°C

[1] B C PERSSON  C G  G J?ger. The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2’-O-methyltransferase activity.[J]. Nucleic Acids Research, 1997, 25 20: 4093-4097. DOI: 10.1093/nar/25.20.4093
[2] RYOTA YAMAGAMI. Folate-/FAD-dependent tRNA methyltransferase from Thermus thermophilus regulates other modifications in tRNA at low temperatures[J]. Genes to Cells, 2016, 21 7: 740-754. DOI: 10.1111/gtc.12376
[3] JAUNIUS URBONAVICIUS  Glenn R B  Jér?me M B Durand. Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri.[J]. Journal of Bacteriology, 2002, 184 19: 5348-5357. DOI: 10.1128/jb.184.19.5348-5357.2002
[4] MIROS?AWA Z. BARCISZEWSKA  Jan B  Tamara D Mashkova. The primary structure of lupin seed 5.8 S ribosomal RNA[J]. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990, 1049 3: Pages 343-345. DOI: 10.1016/0167-4781(90)90108-e
[5] K SIRUM-CONNOLLY  T L M. The role of nucleotide modifications in the yeast mitochondrial ribosome.[J]. Nucleic acids symposium series, 1995, 33: 73-75.
[6] TSUNEATSU MORI . Isolation and Identification of Apoptosis Inducing Nucleosides from CD57+HLA-DRbrightNatural Suppressor Cell Line[J]. Biochemical and biophysical research communications, 1998, 251 2: Pages 416-422. DOI: 10.1006/bbrc.1998.9423