20325-40-0

Off-white powder.

3,3'-DIMETHOXYBENZIDINE DIHYDROCHLORIDE(20325-40-0) is an ether substituted acidic organic salt. Reacts weakly as a acid to neutralize bases. May react with strong oxidizing agents or strong reducing agents.

Water soluble.

Flash point data are not available for this chemical, but 3,3'-DIMETHOXYBENZIDINE DIHYDROCHLORIDE is probably combustible.

o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid (Item No. 16219), and glucose. It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites. This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.

Off-white or grey tablets

It is suitable for use in ELISA procedures. In neurohistochemistry o-Dianisidine dihydrochloride has demonstrated perikaryal labeling as well as retrograde and anterograde transport to the primary olfactory cortex. It has also been used for a paper based-micro fluidic device to semi-quantitatively measure lactate, uric acid, and glucose. 3,3'-Dimethoxybenzidine is used principally as an intermediate in the production of commercial bisazobiphenyl dyes for coloring textiles, paper, plastic, rubber, and leather.

[1] MAMORU SUGIURA  Kazuyuki H. A new colorimetric method for determination of serum glucose[J]. Clinica Chimica Acta, 1977, 75 3: Pages 387-391. DOI: 10.1016/0009-8981(77)90357-6
[2] M M MESULAM  D L R. Sensitivity in horseradish peroxidase neurohistochemistry: a comparative and quantitative study of nine methods.[J]. Journal of Histochemistry & Cytochemistry, 1979, 27 3: 763-773. DOI: 10.1177/27.3.113450
[3] 3,3’-Dimethoxybenzidine and dyes metabolized to 3,3’-dimethoxybenzidine: 3,3’-dimethoxybenzidine.[J]. Report on carcinogens?: carcinogen profiles, 2011, 12: 164-165.