1783-96-6

6384-18-5

1783-96-6

In a reaction vessel, 4.85 g (0.03 mol) of dimethyl (S)-2-aminosuccinate was added to 50 mL of 6 M potassium hydroxide solution and the hydrolysis reaction was carried out by heating and refluxing for 3 hrs. Upon completion of the reaction, the pH of the reaction solution was adjusted to 6.5 with 6 N hydrochloric acid.The reaction solution was purified by passing it through a regenerated cation exchange resin column and the effluent with pH=4 was collected. The collected effluent was concentrated under reduced pressure to one-fifth of the original volume and then precipitated by adding 95% methanol. The precipitate was collected by filtration and dried to give 3.3 g of D-aspartic acid in 82.5% yield.