ChemicalBook CAS DataBase List 1320-06-5

1320-06-5

Name	Solvent Red 27
CAS	1320-06-5
EINECS(EC#)	215-295-3
Molecular Formula	C26H24N4O
MDL Number	MFCD00003898
Molecular Weight	408.49
MOL File	1320-06-5.mol

Synonyms

1-[2,5-DIMETHYL-4-(2,5-DIMETHYLPHENYLAZO)PHENYLAZO]-2-NAPHTHOL 1-([4-(XYLYLAZO)XYLYL]AZO)-2-NAPHTHOL CERES RED 5B CI 26125 CI NO 26125 FAT RED 5B OIL RED O OIL RED O SOLUTION SOLVENT RED 27 SUDAN RED 5B 1-[[4-[(dimethylphenyl)azo]dimethylphenyl]azo]-2-naphthaleno 1-[[4-[(dimethylphenyl)azo]dimethylphenyl]azo]-2-Naphthalenol abcoloilredo Oil red O(C.1.26125) oil red O electrophoresis 1-[[4-[(dimethylphenyl)azo]dimethylphenyl]azo]-2-naphthol OIL RED O, BIOLOGICAL STAIN, CERTIFIED OIL RED O ELECTROPHORESIS REAGENT OIL RED O SUDAN RED 5B HIGH PURITY GRADE C.I. 26125 2-Naphthalenol, 1-4-(dimethylphenyl)azodimethylphenylazo-

Chemical Properties

Appearance	dark green to dark brown crystalline powder
Melting point	120 °C (dec.)(lit.)
bulk density	270kg/m3
Fp	103℃
storage temp.	Store at RT.
solubility	chloroform/ethanol (1:1): soluble1mg/mL
Colour Index	26125
form	powder
color	Red to dark greenish-brown
Water Solubility	Soluble in ethanol:chloroform (1:1). Insoluble in water.
λmax	518 nm, 359 nm
BRN	3491382
Biological Applications	Detecting early stages of atherosclerotic lesion formation; treating obesity,hyperlipidemia,fatty liver,leptin production disorders; dental impression material; measuring phagocytosis
InChI	InChI=1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3/b28-27+,30-29+
InChIKey	NPGIHFRTRXVWOY-XOXGWFOHSA-N
SMILES	C12C=CC=CC1=CC=C(O)C=2/N=N/C1C=C(C(/N=N/C2C=C(C=CC=2C)C)=CC=1C)C
CAS DataBase Reference	1320-06-5(CAS DataBase Reference)
EPA Substance Registry System	1320-06-5(EPA Substance)

Safety Data

Symbol(GHS)	GHS02,GHS07
Signal word	Danger
Hazard statements	H225-H319-H336
Precautionary statements	P210-P305+P351+P338
Hazard Codes	F,Xi
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S22:Do not breathe dust . S24/25:Avoid contact with skin and eyes . less more
RIDADR	UN1219 - class 3 - PG 2 - Isopropanol, solution
WGK Germany	3
TSCA	Yes
HazardClass	3
HS Code	32041900
Storage Class	11 - Combustible Solids

Hazard Information

Physical properties

Solvent Red 27 is a dark green to dark brown crystalline powder.

Uses

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.

Uses

Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.

Definition

ChEBI: Oil red O is a bis(azo) compound that is 2-naphthol substituted at position 1 by a {4-[(2,5-dimethylphenyl)diazenyl]-2,5-dimethylphenyl}diazenyl group. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of naphthols. It is functionally related to a 2-naphthol.

Preparation

2-Amino-2′,3,4′,5-tetramethylazobenzene diazotization, and Naphthalen-2-ol coupling.

General Description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Properties and Applications

blue light red. Soluble in ethanol for yellow red, soluble in acetone, soluble in benzene. In concentrated sulfuric acid to green light blue, blue purple precipitation after dilution.

Standard	Light Fastness	Heat-resistant(℃)		water	Sodium Carbonate(5%)	Hydrochloric acid(5%)
		Melting point	Stable
ISO	Well	108-120	70≥ 200 Sublimation	Insoluble	No change	No change

Material Safety Data Sheet(MSDS)

Questions And Answer

Spectrum Detail

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