Physical properties
Solvent Red 27 is a dark green to dark brown crystalline powder.
Uses
Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.
Uses
Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.
Definition
ChEBI: Oil red O is a bis(azo) compound that is 2-naphthol substituted at position 1 by a {4-[(2,5-dimethylphenyl)diazenyl]-2,5-dimethylphenyl}diazenyl group. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of naphthols. It is functionally related to a 2-naphthol.
Preparation
2-Amino-2′,3,4′,5-tetramethylazobenzene diazotization, and Naphthalen-2-ol coupling.
General Description
Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.
Properties and Applications
blue light red. Soluble in ethanol for yellow red, soluble in acetone, soluble in benzene. In concentrated sulfuric acid to green light blue, blue purple precipitation after dilution.
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Standard
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Light Fastness
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Heat-resistant(℃)
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water
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Sodium Carbonate(5%)
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Hydrochloric acid(5%)
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Melting point
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Stable
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ISO
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Well
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108-120
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70≥ 200
Sublimation
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Insoluble
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No change
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No change
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