Тритон X-100 (Triton?? X-100)

Использование

Triton^? X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

Общее описание

Octophenolpoly(ethyleneglycolether)_n
Especially used for purification in membrane research
Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.

Профиль безопасности

Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes.