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How to prepare ACES Buffer

Jan 10,2024

ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid. ACES is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good buffers developed in the 1960s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. ACES is very soluble in water and delivers high clarity in solutions. ACES also works well as a buffer in the isoelectric focusing of proteins. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. This particular product was prepared in 3D water and filtered through a 0.22 � filter.

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ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe:

Prepare 800 mL of dH2O in a suitable container.

Add 18.22 g of ACES to the solution.

Adjust solution to the desired pH by 10N NaOH.

Add dH2O until the volume is 1 L.

7365-82-4 ACES Bufferprepare 6.7 pHRecipezwitterionic buffer ACES
7365-82-4

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