腔肠素

Coelenterazine is the luciferin, the light-emitting molecule, found in many aquatic organisms across seven phyla. It is the substrate of many luciferases such as Renilla reniformis luciferase (Rluc), Gaussia luciferase (Gluc), and photoproteins, including aequorin, and obelin. Coelenterazine was used: (1) For Aequorin assays; (2) For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells2; (3). In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity. Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca2+ binds to the complex; used to measure Ca2+ concentration in cells with high sensitivity and large dynamic range.