腔肠素|T6808|TargetMol

Coelenterazine
55779-48-1

￥987 1mg 起订

￥1570 2mg 起订

￥2970 5mg 起订

上海更新日期：2024-09-24

TargetMol中国（陶术生物）

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联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 腔肠素

英文名称：: Coelenterazine

CAS号：: 55779-48-1

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.02%

产品类别：: 抑制剂

货号：: T6808

Product Introduction

Bioactivity

Name	Coelenterazine
Description	Coelenterazine is a superoxide anion-sensitive chemiluminescent probe that can be used to detect the chemiluminescence of peroxynitrite and can serve as a luminescent enzyme substrate for apoaequorin and Renilla luciferase.
Cell Research	Cells are plated at a density of 5×10^4?cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2?and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only.
Animal Research	Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only.
In vitro	在监测多种生物学过程时，coelenterazine (CTZ) 可以被海葵 Renilla reniformis (Rluc) 和海洋桡足类 Gaussia princeps (Gluc) 等luciferases 催化，产生蓝色光线发射。在表达Gli36-Gluc或Gli36-Rluc的Gli36细胞中，coelenterazine展现出生物发光信号。[1] 在RIN-5F、4T1、HCT 116、HEK 293、293FT、HeLa 和 INS-1细胞中，coelenterazine可用于检测超氧阴离子浓度。[2]
In vivo	方法：将 Gli36-Gluc 和 Gli36-Rluc 细胞注射到裸鼠的腿部肌肉中小鼠静脉注射100μg（4mg / kg体重）Coelenterazine，并立即使用冷却电荷耦合器件（CCD）相机成像；向上述小鼠注射了500μg（20mg / kg）水溶性Coelenterazine，并使用CCD相机对它们进行成像。结果：水溶性Coelenterazine对Gluc的光子数高出10倍，对Rluc的光子数高出4倍，表明s-Coelenterazine在体内检测腔肠酸荧光素酶方面更敏感。[1]
Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	Ethanol : 1 mg/mL (2.36 mM), DMSO inactivates the activity of Coelenterazine. H2O : < 1 mg/mL (insoluble or slightly soluble)
Keywords	inhibit \| Inhibitor \| Coelenterazine

腔肠素|||Oplophorus luciferin|||CTZ|||Coelenteramine|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子