Lactase is also named β-galactosidase, derived from the Aspergillus oryzae, it’s a food grade enzyme which produced from submerged fermentation. It’s widely used in the dietary supplements as a digestive aid to reduce the lactose intolerance.
Action Principle
The lactase can hydrolyze the β-glycosidic bond of lactose molecule into glucose and galactose.
Product Characteristic
Temperature range: 5℃~65℃ optimum temperature: 55℃~60℃
Product appearance: white to light brown powder, the colour may vary from batch to batch.
Product odor: slight odor of fermentation Standard enzyme activity: no less than 100000 ALU/g
Enzyme activity definition: One lactase unit is defined as the quantity of enzyme that will liberate o-nitrophenol at a rate of 1µmol per minute under the condition of hydrolyze oNPG at 37℃ and pH 4.5.
Product standard: GB1886.174<< enzymes for food industry >>
Packaging and Storage
Packaging: 25kg/drum
Storage: store at room temperature, away from light, cold storage is better.
Shelf life: 2 years.
Why we use the Lactase
Natural lactase usually present in the human gut. Were very low, however, when people ate dairy, because most of the human body, a lack of lactase, so difficult for them to digest and absorb the milk sugar lactose, the lactose in milk can not be decomposed use, intestinal bacteria break down the fermentation, producing a large carbon dioxide gas, resulting in intestinal expansion and peristalsis contraction to strengthen, resulting in bowel, diarrhea, bloating and other symptoms of this disease is called lactose intolerance. Lactase deficiency worldwide is widespread, especially in Asia, Africa accounted for a large proportion of our country is also a common phenomenon.
Mechanisms
α-Galactoside (such as raffinose and stachyose) is a kind of anti-nutrient factor that
exists in some plant seeds, whose content is highest in bean seed while lowest in
grain seed. Poultry has no endogenous enzyme to digest α-galactosidase. Pig can
partially ferment it with the aid of intestinal microoganism and yield CO2 and NH3 at
the same time, which arouses flatulence, nausea, borborygmus and diarrhea. Thus
α-galactoside wins a name for flatulence factor. α-Galactoside can be gathered
massively in the body of livestock and poultry, which will influence on the breeding
effect of feed and organism while reaching a certain amount.
When people consumed the dairy products or lactose-containing food, as a lack of lactase in the body, the lactose is digested and fermented by intestinal bacteria and forms a mass of carbon dioxide gas. It can lead to the expansion of intestinal tract and the strengthening of intestinal peristalsis. It brings borborygmus, diarrhea, abdominal distension and so on. It is called lactose intolerance.
The Lactase from Aspergillus oryzae has a high activity at low pH(pH3.5~5.5) making it particularly suitable for digest lactose in the stomach, and it’s therefore ideal for use in the dietary supplements as digestive aid.
The dose of fungal lactase depends on the amount of the lactose and the size of meal consumed, one general guideline is 2,500 ALU per 10~13 grams of lactose consumed. We recommend a dose of 4,000~6,000 ALU with each lactose-containing meal. For very sensitive lactose-intolerant consumers (need a 100% conversion), 10,000 ALU per meal is recommended.
Other application: Apply to process free-lactose yoghurt.
Content of α-galactoside in common plant feed stuff
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Plant seed
Raffinose(%)
Stachyose(%)
Total
Soy bean
1.21
4.22
5.43
Cottonseed
6.91
2.36
9.27
Peanut
0.33
0.99
1.32
Sunflower seed
3.09
0.14
3.23
Wheat
0.70
0.70
Barley
0.71
0.71
Corn
0.26
0.26
α-Galactosidase can digest anti-nutrient factors, such as raffinose and stachyose, and galactosidase. It can also hydrolyze galactomannans contained in bean gum with mannase to achieve synergy.