Smac/Diablo抗体;Smac/Diablo Antibody—艾普蒂 新品

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     Western blot analysis of Smac/Diablo expression in Jurkat cell lysate.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human kidney, using Smac/Diablo Antibody.    

  
  

**Background of Smac/Diablo Antibodies**

Smac (Second mitochondria-derived activator of caspases), also known as Diablo (Direct IAP-binding protein with low pI), is a mitochondrial protein that plays a critical role in apoptosis. Upon apoptotic stimuli, Smac/Diablo is released into the cytosol, where it neutralizes inhibitor of apoptosis proteins (IAPs) such as XIAP, thereby promoting caspase activation and programmed cell death. Its function is tightly linked to the mitochondrial pathway of apoptosis, making it a key focus in cancer research, particularly in understanding apoptosis resistance in tumors.

Antibodies targeting Smac/Diablo are essential tools for studying its expression, localization, and interaction with IAPs. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous Smac/Diablo levels in cells or tissues. Researchers also employ them to investigate how Smac/Diablo release is regulated under stress conditions or during therapeutic treatments (e.g., chemotherapy or radiation). Specific antibodies can distinguish between the full-length mitochondrial form and the processed mature form, aiding in studies of its activation mechanism.

Furthermore, Smac/Diablo antibodies contribute to developing cancer therapies, such as Smac mimetics, which mimic its IAP-binding domain to sensitize resistant tumors to apoptosis. Validation of these antibodies (e.g., via knockout cell lines) ensures specificity, critical for accurate interpretation of experimental data. Overall, Smac/Diablo antibodies are vital for unraveling apoptosis-related pathways and advancing therapeutic strategies in oncology.