重组人SMAC/DIABLO蛋白-ACROBiosystems百普赛斯

SMAC产品信息

英文名称：Diablo homolog, mitochondrial

中文名称：DIABLO蛋白

靶点别称：SMAC

物种：Human

标签：His Tag

属性：Protein

标记：Unconjugated

表达区间及表达系统（Source）

Human SMAC, His Tag (DIO-H5129) is expressed from E. coli cells. It contains AA Ala 56 - Asp 239 (Accession # NP_063940).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.7 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Diablo同源物线粒体（Diablo）也被称为低pI的直接IAP结合蛋白和第二线粒体衍生的胱天蛋白酶激活剂（SMAC），可以与BIRC2/c-IAP1、BIRC3/c-IAP2、XIAP/BIRC4、BIRC6/bruce和BIRC7/livin相互作用。通过对抗凋亡蛋白抑制剂（IAP）的抑制活性，DIABLO还可以通过激活细胞色素c/Apaf-1/caspase-9通路中的胱天蛋白酶来促进凋亡。此外，DIABLO通过抑制BIRC6/bruce与胱天蛋白酶的结合来抑制其活性。此外，DIABLO在下调XIAP/BIRC4丰度的能力方面存在缺陷。