DIABLO抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).    

  
  

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     mmunohistochemical analysis of paraffin-embedded human pancreas tissues using PSAP mouse mAb with DAB staining.    

  
  

The DIABLO (Direct IAP-Binding Protein with Low pI) protein, also known as Smac (Second mitochondria-derived activator of caspases), is a mitochondrial pro-apoptotic factor critical for regulating programmed cell death. It is released into the cytosol during apoptosis, where it neutralizes inhibitor of apoptosis proteins (IAPs) by binding to them, thereby relieving their suppression of caspase activity. This interaction promotes caspase activation and execution of apoptosis.

Antibodies targeting DIABLO/Smac are essential tools for studying its expression, localization, and function in cellular pathways. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess DIABLO levels in cancer research, as dysregulation of DIABLO is linked to tumor progression and chemoresistance. Reduced DIABLO expression in some cancers correlates with evasion of apoptosis, making it a potential biomarker or therapeutic target.

Additionally, DIABLO antibodies aid in exploring its role in neurodegenerative diseases and ischemic injuries, where impaired apoptosis regulation contributes to pathology. The development of SMAC mimetics—small molecules mimicking DIABLO’s IAP-binding function—highlights its therapeutic relevance. These mimetics, combined with antibody-based studies, advance understanding of apoptosis mechanisms and strategies to restore cell death sensitivity in treatment-resistant diseases. Research on DIABLO continues to uncover its broader roles in cellular stress responses and immune regulation.