ML-7盐酸盐|T3050|TargetMol

ML-7 hydrochloride
110448-33-4

￥125 1mg 起订

￥241 5mg 起订

￥433 10mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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联系人：邵小姐

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邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: ML-7盐酸盐

英文名称：: ML-7 hydrochloride

CAS号：: 110448-33-4

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.61%

产品类别：: 抑制剂

货号：: T3050

Product Introduction

Bioactivity

Name	ML-7 hydrochloride
Description	ML-7 hydrochloride (ML-7 HCl) is a cell-permeable, reversible, effective, ATP-competitive, and specific inhibitor of myosin light chain kinase (Ki: 300 nM); also inhibits smooth-muscle myosin light chain kinase, PKA, and PKC.
Cell Research	Cells are treated with vehicle alone, Blebbistatin, ML-7, ML-9, latrunculin A or Y-27632 in media without serum at varying concentrations and time points before being fixed or prepared for FACS analysis. Untreated cells are also serum starved for the duration of the inhibitor treatment. Stock solutions of each agent are made in DMSO (50 μM Blebbistatin, 5 mM LA), 50% ethanol (10 mM ML-7), 70% ethanol (20 mM ML-9) or water (20 mM Y-27632) and are maintained at -20°C.(Only for Reference)
Kinase Assay	IKK-2 Assay: Recombinant human IKK-2 (residues 1-756) is expressed in baculovirus as an N-terminal GST-tagged fusion protein, and its activity is assessed using a time-resolved fluorescence resonance energy transfer assay. In brief, IKK-2 (5 nM final) diluted in assay buffer (50 mM HEPES, 10 mM MgCl2, 1 mM CHAPS, pH 7.4, with 1 mM DTT and 0.01% w/v BSA) is added to wells containing various concentrations of compound or dimethyl sulfoxide (DMSO) vehicle (3% final). The reaction is initiated by the addition of GST-IκBα substrate (25 nM final)/ATP (1 μM final), in a total volume of 30 μL. The reaction is incubated for 30 min at room temperature, then terminated by the addition of 15 μL of 50 mM EDTA. Detection reagent (15 μL) in buffer (100 mM HEPES, pH 7.4, 150 mM NaCl, and 0.1% w/v BSA) containing antiphosphoserine- IκBα-32/36 monoclonal antibody 12C2, labeled with W-1024 europium chelate, and an allophycocyanin-labeled anti-GST antibody is added, and the reaction is further incubated for 60 min at room temperature. The degree of phosphorylation of GST- IκBαis measured as a ratio of specific 665-nm energy transfer signal to reference europium 620-nm signal, using a Packard Discovery plate reader.
In vitro	ML-7通过抑制MLCK激活粘附性MCF-10A及其Ras变异细胞中的caspase-3[2]。ML-7处理以剂量依赖的方式降低MLC20磷酸化水平，并相应增加平滑肌细胞(SMC)的死亡率。ML-7对MLCK的抑制作用具有高度选择性，其对MLCK的Ki为0.3 μM，而对蛋白激酶A的Ki为21 μM，对蛋白激酶C的Ki为42 μM。过表达Bcl-2可以保护细胞免受ML-7引起的凋亡[4]。
In vivo	ML7通过调节高脂饮食喂养的兔子中的MLCK和MLC磷酸化作用，影响紧密连接（TJ）蛋白区域桥蛋白（ZO)-1和闭锁蛋白的表达，从而改善血管内皮功能障碍（VED）和动脉粥样硬化（AS）。ML7能降低高脂饮食兔子动脉壁中MLCK和MLC的磷酸化表达，并减少AS兔子中的脂质沉积损伤[1]。
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 60 mg/mL (132.53 mM), Sonication is recommended.
Keywords	ML-7 Hydrochloride \| Inhibitor \| Yes-associated protein \| inhibit \| Myosin \| ML-7 \| ML7 hydrochloride \| ML7 \| ML7 Hydrochloride \| YAP \| ML 7 \| ML 7 hydrochloride \| ML 7 Hydrochloride
Inhibitors Related	JAK1/2/3 Inhibitor 1 \| Camostat mesylate \| AICAR \| FOY 251 \| Acefylline \| Benzamidine hydrochloride \| Staurosporine \| α-Vitamin E \| Nafamostat mesylate
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Anti-Cancer Compound Library \| Bioactive Compounds Library Max \| Cell Cycle Compound Library \| Kinase Inhibitor Library \| Anti-Aging Compound Library \| Stem Cell Differentiation Compound Library \| TGF-beta/Smad Compound Library \| Tyrosine Kinase Inhibitor Library

ML-7盐酸盐|||ML-7 HCl|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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