盐酸多西环素|T1140|TargetMol

Doxycycline hydrochloride
10592-13-9

￥183 5mg 起订

￥258 10mg 起订

￥413 25mg 起订

上海更新日期：2025-11-17

TargetMol中国（陶术生物）

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联系人：邵小姐

手机：15002134094 拨打

邮箱：marketing@targetmol.cn

产品详情:

中文名称：: 盐酸多西环素

英文名称：: Doxycycline hydrochloride

CAS号：: 10592-13-9

品牌：: TargetMol

产地：: 美国

保存条件：: store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

纯度规格：: 98.77%

产品类别：: 抑制剂

货号：: T1140

Product Introduction

Bioactivity

名称	Doxycycline hydrochloride
描述	Doxycycline hydrochloride (Hyclate) is an orally active tetracycline antibiotic with broad-spectrum matrix metalloproteinase (MMP) inhibitory activity, possessing both antibacterial and anti-cancer cell proliferation effects, and can be used to induce gene expression in ON-OFF systems.
细胞实验	Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-H3]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA.
激酶实验	Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size.
体外活性	在Wistar大鼠中,每天 Doxycycline（25 mg）能够阻断主动脉弹性蛋白的结构性恶化,降低动脉壁中基质金属蛋白酶MMP-2和MMP-9的活性,从而减少平滑肌细胞在内膜的复制.
体内活性	在类风湿滑膜成纤维细胞和内皮细胞中,Doxycycline（50 μM）下调MMP-8的在mRNA和蛋白水平的表达,同时完全抑制II型胶原的降解。在角膜上皮细胞中,Doxycycline抑制脂多糖诱导的IL-1β的mRNA和蛋白的量的增加。
存储条件	store under nitrogen \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
溶解度	DMSO : 10 mM, Sonication is recommended.
关键字	U343 \| SVG \| MMP \| MCF 12A \| Matrix metalloproteinases \| LNT-229 \| Inhibitor \| inhibit \| G55 \| Doxycycline hydrochloride \| Doxycycline Hydrochloride \| Doxycycline \| Bacterial \| anti-proliferative activity \| Antibiotic \| antibacterial activity \| 293T
相关产品	Adipic dihydrazide \| Trisodium Phosphate \| Doxycycline \| Neomycin sulfate \| BES \| Metronidazole \| Terbinafine hydrochloride \| Ampicillin sodium \| Copper(Ⅱ) Sulfate \| Acetic Acid \| Sulfamethoxazole sodium \| Kanamycin sulfate
相关库	抗癌活性化合物库 \| 抗生素库 \| 临床失败化合物库 \| 上市药物库

盐酸多西环素|||Vibramycin|||Hyclate|||Doxylin|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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