西地那非柠檬酸盐|T0467|TargetMol

Sildenafil citrate
171599-83-0

￥153 5mg 起订

￥198 10mg 起订

￥318 25mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

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联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 西地那非柠檬酸盐

英文名称：: Sildenafil citrate

CAS号：: 171599-83-0

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.59%

产品类别：: 抑制剂

货号：: T0467

Product Introduction

Bioactivity

名称	Sildenafil citrate
描述	Sildenafil citrate (UK-92480 citrate) , a cyclic guanosine monophosphate (cGMP)-specific phosphodiesterase type 5 (PDE5) Inhibitor, is used extensively for erectile dysfunction and less commonly for pulmonary hypertension.
细胞实验	Sildenafil is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. Cells at approximately 90% confluence are harvested with 0.1% trypsin/0.01% ethylene diamine tetraacetic acid (EDTA) solution and seeded into a 96-well plate at a density of 2×104 cells/well and grown in RPMI-1640 containing 10% FBS for three days, followed by serum starvation for three days. Cells are then incubated for different time with various concentration of serotonin or 1 μM Sildenafil followed by serotonin with or without U0126, as indicated. Control cells are treated in the same way except sterile PBS replaced the drug. After treatment, medium is changed to fresh medium, and cells are incubated with 5 g/L of MTT for four hours. MTT is then dissolved with 150 μL of 10% DMSO for 20 minutes. The optical densities (OD) in the 96-well plates are determined using a microplate reader at 570 nm[2].
激酶实验	COX enzyme assay in vitro: Expression of COX protein in insect cells is determined by assessing PG-synthetic capability in homogenates from cells incubated for 3 days with COX-1 or COX-2 baculovirus. Cells expressing COX-1 or COX-2 are homogenized and incubated with arachidonic acid (10 μM). COX activity is determined by monitoring PG production. No COX activity is detected in mock-infected Sf9 cells. Celecoxib are preincubated with crude 1% CHAPS homogenates (2-10 μg of protein) for 10 minutes before addition of arachidonic acid. PGE2 formed is detected by ELISA after 10 minute incubation.
体外活性	在麻醉的狗体内,Sildenafil citrate增强盆骨神经刺激下阴茎的勃起功能.在Sprague-Dawley大鼠体内, 每天Sildenafil（20 mg/kg）以时间剂量依赖的方式改善勃起功能,同时Sildenafil明显减少细胞凋亡指数,并且增加akt 和eNOS的磷酸化作用.
体内活性	在内皮细胞中,Sildenafil通过降低由高血糖引起的氧化应激和增加细胞内Ca2 +水平,改善胰岛素信号传导NO产生。Sildenafil citrate是一种强效的PDEⅤ型的可逆选择性抑制剂,其能够有效阻断cGMP的水解作用(Ki ～3 nM)。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 45 mg/mL (67.5 mM)
关键字	inhibit \| UK92480 \| UK-92480 Citrate \| UK-92480 \| UK 92480 Citrate \| Apoptosis \| Sildenafil citrate \| Inhibitor \| Autophagy \| UK 92480 \| Phosphodiesterase (PDE) \| Sildenafil \| Sildenafil Citrate \| UK92480 Citrate
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西地那非柠檬酸盐|||枸橼酸西地那非|||UK-92480 citrate|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子