化合物 Torin 2|T6100|TargetMol

Torin 2
1223001-51-1

￥239 1mg 起订

￥333 2mg 起订

￥533 5mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 化合物 Torin 2

英文名称：: Torin 2

CAS号：: 1223001-51-1

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.32%

产品类别：: 抑制剂

货号：: T6100

Product Introduction

Bioactivity

Name	Torin 2
Description	Torin 2(IC50=0.25 nM), a specific and effective mTOR inhibitor, is the 800-fold greater specific activity for mTOR than PI3K and improves pharmacokinetic properties. The EC50 of Torin 2 for ATM/ATR/DNA-PK inhibition is 28 nM/35 nM/118 nM, respectively.
Cell Research	For viability, MZ-CRC-1 and TT cells are seeded in quadruplicate in 96-well plates (1.0×104 cells per well) in culture media with 2.5% and 4% FBS, respectively. After 24 hours, cells are treated with Torin 2. At the indicated time point, cells are incubated for 3 hours with 10 μL of CellTiter96 AQueous One solution in 100 μL of culture media and absorbance is measured at 490 nm.(Only for Reference)
Kinase Assay	mTOR and PI3K Cellular Assays: Cellular IC50 values for mTOR are determined using p53?/? MEFs. Cells are treated with vehicle or increasing concentrations of Torin 2 for 1 h and then lyse. Phosphorylation of S6K1 Thr-389 is monitored by immunoblotting using a phospho-specific antibody. Meanwhile, cellular IC50 values for PI3Ka are determined based on phosphorylation of Akt Thr-308 in p53?/?/mLST8?/? MEFs or human PC3 cells expressing the S473D mutant of Akt1.
In vitro	在Th-MYCN 小鼠体内,Torin 2（20 mg/kg）消除MYCN肿瘤,减少MYCN蛋白质水平,并诱导细胞凋亡.在小鼠肝微粒体稳定性试验中,Torin 2表现出>95%的药效学响应,半衰期为11.7分钟.在雄性Swiss albino小鼠中,静脉内或口服Torin 2后,体内表现出最好的生物利用度为51%,半衰期为0.72小时,和低清除率是19.6 mL/min/kg.
In vivo	在MZ-CRC-1和TT细胞中, Torin 2（< 50 nM）引起细胞的的活性明显减少,Torin 2（100 nM）使细胞的迁移明显减少。Torin 2抑制mTORC1,因此通过促进其核转运激活TFEB,EC50为1.666 mM。Torin 2与PI3Kγ具有相同的结合模式,V882作为铰链结合点,在内部疏水袋Y867,D841 和D964再提供三个作用于氨基比林侧链的氢键,类似于mTOR的Y2225,D2195 和D2357。
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 1 mg/ml, Sonication is recommended.
Keywords	DNA-dependent protein kinase \| mTOR \| Autophagy \| Torin-2 \| inhibit \| Mammalian target of Rapamycin \| DNA-PK \| Torin2 \| Inhibitor \| Torin 2 \| Apoptosis
Inhibitors Related	Guanidine hydrochloride \| Naringin \| Taurine \| Gefitinib \| Hydroxychloroquine \| 5-Fluorouracil \| Curcumin \| Stavudine \| Tributyrin \| L-Ascorbic acid \| Paeonol \| Sodium 4-phenylbutyrate
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Kinase Inhibitor Library \| Fluorochemical Library \| Anti-Aging Compound Library \| Highly Selective Inhibitor Library \| Anti-Liver Cancer Compound Library \| Tyrosine Kinase Inhibitor Library \| Reprogramming Compound Library

Torin 2|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子