坦螺旋霉素|T6290|TargetMol

Tanespimycin
75747-14-7

￥341 5mg 起订

￥530 10mg 起订

￥873 25mg 起订

上海更新日期：2026-01-06

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 坦螺旋霉素

英文名称：: Tanespimycin

CAS号：: 75747-14-7

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

纯度规格：: 99.56%

产品类别：: 抑制剂

货号：: T6290

Product Introduction

Bioactivity

名称	Tanespimycin
描述	Tanespimycin (KOS 953) is an Hsp90 inhibitor (IC50=5 nM) and is selective. Tanespimycin depletes intracellular STK38/NDR1 and decreases STK38 kinase activity. Tanespimycin also downregulated stk38 gene expression.
细胞实验	Cells were seeded in 96-well plates at 2,000 cells per well in a final culture volume of 100 μl for 24 h before the addition of increasing concentrations of 17-AAG that was incubated for 5 days. Viable cell number was determined using the Celltiter 96 AQueous Nonradioactive Cell Proliferation Assay. The value of the background absorbance at 490 nm (A490) of wells not containing cells was subtracted. Percentage of viable cells ? (A490 of 17-AAG treated sample/A490 untreated cells) × 100. The IC50 was defined as the concentration that gave rise to 50% viable cell number [1].
激酶实验	Purified native Hsp90 protein or cell lysates in lysis buffer (20 mM HEPES, pH 7.3, 1 mM EDTA, 5 mM MgCl2, 100 mM KCl) were incubated with or without 17-AAG for 30 min at 4 °C, and then incubated with biotin-GM linked to streptavidin magnetic beads for 1 h at 4 °C. Tubes were placed on a magnetic rack, and the unbound supernatant removed. The magnetic beads were washed three times in lysis buffer and heated for 5 min at 95 °C in SDS–PAGE sample buffer. Samples were analyzed on SDS protein gels, and western blots done using indicated antibodies. Bands in the western blots were quantified, and the percentage inhibition of binding of Hsp90 to the biotin-GM was calculated. The IC50 reported is the concentration of 17-AAG needed to cause half-maximal inhibition of binding. For in vitro reconstitution, 5 μM of purified Hsp90 was combined with 1 μM each of Hsp70, Hsp40, p23, and Hop purified proteins [1].
动物实验	B10.BR mice were inoculated with 5×10^5 lymphoma cells through intraperitoneal injection. Seven days following tumor implantation, the mice were I.P. injected with 17-AAG or vehicle (10% DMSO + 40% Cremophor EL: Ethanol (3:1) (v/v) + 50 % PBS) every other day for three weeks. At the cessation of treatment, mice were monitored up to 80 days post tumor cell injection. To determine the effects of 17-AAG on lymphoma initiation in vivo, secondary B10.BR recipient mice were implanted by intraperitoneal injection of 1×10^5 lymphoma cells from the spleens of first-round mice that had been treated with 17-AAG or vehicle. These mice were followed up to 160 days post tumor cell injection to monitor differences in tumor initiation between the mice [4].
体外活性	方法：人A-431、A549、BGC-823、HepG2、HUVEC、L02、MDA-MB-231细胞用Tanespimycin（0-10 μM）处理72小时，使用MTT方法检测细胞生长抑制情况。结果：Tanespimycin抑制A-431（IC50=89 nM）、A549（IC50=81 nM）、BGC-823（IC50=847 nM）、HepG2（IC50=91 nM）、HUVEC（IC50=282 nM）、L02（IC50=99 nM）、MDA-MB-231（IC50=0.28 μM）细胞生长。[1] 方法： CCA 细胞用Tanespimycin（0.6 μM）处理72小时，使用Western Blot方法检测靶蛋白表达水平。结果：Tanespimycin下调 Bcl-2、Survivin 和 Cyclin B1，上调 cleaved PARP。[2]
体内活性	方法：为研究Tanespimycin的抗肿瘤活性，将Tanespimycin（5-40 mg/kg）每隔一天腹腔注射给接种淋巴瘤小鼠，持续三周。结果：Tanespimycin在体内抑制淋巴瘤。[3]
存储条件	keep away from direct sunlight,keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (1.71 mM), Sonication is recommended. DMSO : 50.5 mg/mL (86.22 mM), Sonication is recommended.
关键字	tumor \| Tanespimycin \| stk38 \| prostate \| NSC-330507 \| NSC330507 \| Mitophagy \| Mitochondrial Autophagy \| KOS-953 \| KOS953 \| Inhibitor \| inhibit \| HSP90 \| HSP \| HER2 \| Heat shock proteins \| CP-127374 \| CP127374 \| cancer \| Bacterial \| Autophagy \| Apoptosis \| Antibiotic \| A549
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坦螺旋霉素|||NSC 330507|||KOS 953|||CP 127374|||17-AAG|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(13年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子