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网站主页 化工产品目录 生物化工 核苷类药物 脱氧核苷酸及其类似物 地西他滨 地西他滨
  • 地西他滨|T1508

地西他滨|T1508

Decitabine
2353-33-5
￥159 1mg 起订
￥335 5mg 起订
￥535 10mg 起订
上海 更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年
联系人：邵小姐
电话：021-021-33632979拨打
手机：15002134094 拨打
邮箱：marketing@targetmol.com

产品详情:

中文名称：
地西他滨
英文名称：
Decitabine
CAS号：
2353-33-5
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.87%
产品类别：
抑制剂
货号：
T1508

Product Introduction

Bioactivity

名称Decitabine
描述Decitabine (Deoxycytidine) is a deoxycytidine analog, a DNA methyltransferase inhibitor with oral activity. Decitabine has antitumor activity and antimetabolic activity. Decitabine induces cell cycle arrest and apoptosis.
细胞实验For cell cycle analysis, KARPAS-299 cells were incubated for 24 h with 1 μM of 5-aza-CdR in RPMI and grown for 4 days in fresh RPMI only. Then, 105–106 cells were suspended in 500 μl PI-buffer (0.1% Na–citrate dihydrate, 0.1% Triton X-100, 0.1% RNAse (DNAse free) in PBS). Propidium–iodide (ROTH, dissolved in PBS) was added to a concentration of 10 μg/ml and the cells were incubated for 30 min at 37 °C. The analysis was performed on a flow cytometer using the BD FACS Diva Software. Three independent samples of 5-aza-CdR treated and PBS controls were analyzed. Descriptive statistics for analysis are reported as mean ± SEM [4].
激酶实验The rate of DNA synthesis was measured by the incorporation of radioactive thymidine into DNA. HL-60 (5 × 10^3 cells/ml) and KG1a cells (10^4 cells/ml) were suspended in 2 ml RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 h (drugs were added simultaneously). At 48 h, 0.5 μCi [3H] thymidine (6.7 Ci/mmol) was added to each well and incubated for an additional 24 h. The cells were placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA were then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using scintillation counter. The IC50 is defined as the concentration of drug that inhibits by 50% the DNA synthesis of the leukemic cell lines from the dose-response curve [1].
动物实验For xenografts, NOD.CB17-Prkdc?scid/NCrHsd (NOD/SCID, Harlan Laboratories) mice were used. KARPAS-299 human cells were grown as described above, dissolved in sterile PBS to a concentration of 1×107 cells/ml and inoculated subcutaneously (1×10^6 cells/injection) into the right and left flanks of the mice. Tumor range was followed measuring tumor length and tumor width with a calliper. Mice weighed approximately 25 g at the beginning of the therapy. 5-Aza-CdR was dissolved in sterile PBS and was administered intraperitoneally (i.p.). Each mouse received 2.5 mg/kg/mouse per treatment. Control mice were administered 100 μl of sterile PBS. Therapies were adjusted regarding start and duration of the treatment in order to obtain optimal treatment procedures. In schedule A, three mice were treated with 5-aza-CdR 11 days after inoculation, when tumor size was approximately 1 cm2. The control group contained two mice. The mice received 5-aza-CdR or PBS every day for eight days. In schedule B, two mice were treated with 5-aza-CdR three days after inoculation and three mice five days after inoculation when tumors were not or just palpable. 5-Aza-CdR was administered every other day for five times to each mouse. The control group contained two mice [4].
体外活性方法：人急性白血病细胞 molt4 用 Decitabine (0.00625-100 μM) 处理 24-96 h，使用 CCK-8 方法检测细胞增殖。 结果：Decitabine 以剂量和时间依赖的方式抑制 molt4 细胞的增殖，处理 72 h 和 96 h 的 IC50 分别为 84.461 μM 和 10.113 μM。[1] 方法：人 BCP-ALL 细胞 SEM 和 RS4;11 用 Decitabine (1000 nM) 处理 72 h，使用 Flow Cytometry 检测细胞周期情况。 结果：Decitabine 引起 SEM 细胞中 G0/G1 停滞。RS4;11 的细胞周期不受 Decitabine 的影响。[2]
体内活性方法：为检测体内抗肿瘤活性，将 Decitabine (0.4 mg/kg) 腹腔注射给携带 ALL 肿瘤 SEM-ffluc-GFP 或 RS4;11-ffluc-GFP 的 NSG 小鼠，每天一次，持续三十天。 结果：Decitabine 显著延迟了 SEM-ffluc-GFP 和 RS4-ffluc 衍生的异种移植物模型中的白血病细胞增殖。[2] 方法：为检测体内抗肿瘤活性，将 Decitabine (0.8 mg/kg) 腹腔注射给携带人胆管癌肿瘤 TFK-1 的 Balb-c nu/nu 小鼠，每天一次，持续十四天。 结果：在 TFK-1 小鼠异种移植物中，Decitabine 延缓了荷瘤小鼠的肿瘤生长并提高了其存活率。[3]
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度H2O : 11.4 mg/mL (50 mM)
DMSO : 55 mg/mL (241.01 mM)
关键字DNA Methyltransferase | DNMTs | NSC-127716 | Apoptosis | inhibit | DNA MTases | NSC127716 | Nucleoside Antimetabolite/Analog | Decitabine | Inhibitor
相关产品L-Glutamic acid | Metronidazole | 5-Fluorouracil | Dextran sulfate sodium salt (MW 4500-5500) | Stavudine | Tributyrin | Myricetin | Sorafenib | L-Ascorbic acid | Acetylcysteine | Salicylic acid | Sodium 4-phenylbutyrate
相关库抑制剂库 | 抗癌上市药物库 | 抗癌活性化合物库 | 经典已知活性库 | 已知活性化合物库 | EMA 上市药物库 | 抗衰老化合物库 | FDA 上市药物库 | 药物功能重定位化合物库 | 抗癌临床化合物库
地西他滨|||NSC 127716|||Deoxycytidine|||Dacogen|||5-Aza-2'-deoxycytidine|TargetMol

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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