单克隆Anti DXD抗体-ACROBiosystems百普赛斯

Monoclonal Anti-DXD&Exatecan Antibody, Mouse IgG1 (MALS verified)

抗体来源（Source）

Monoclonal Anti-DXD&Exatecan Antibody, Mouse IgG1 is recombinantly produced from human 293 cells (HEK293).

亚型（Isotype）

Mouse IgG1/kappa

特异性（Specificity）

Specifically recognizes the target-DXD.

应用（Application）

PK, PD, Immunoassay and ELISA

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Dxd (Exatecan ADC derivative) is a potent DNA topoisomerase I inhibitor with an IC50 of 0.31 uM for targeting ADCs (DS-8201A). Dxd was cytotoxic to human tumor cell lines KPL-4, NCI-N87 and SK-BR, 3 and the IC50s of MDA-MB-468 was 1.43 NM-4.07 nM, while lgGADC (Dxd as payload) had no inhibitory effect on HER2 expression in the 4 cell lines. DS8201a (Dxd as payload) had a significant inhibitory effect on HER2-positive KPL-4 and NCI cell lines N87 and SK-BRwith IC50 values of 26.8, 25.4 and 6.7 ng/mL, respectively, but had no inhibitory effect on MDA MB-468 (IC50, > 10000 - mL). HRP-anti-DXD-antibody is a conjugation product of HRP and anti-DXD-antibody. It can be used in PK, PD analysis and ELISA.