PNGase F酶-ACROBiosystems百普赛斯

PNGase F产品信息

别称：PNGase F,PNGase F,PNGF

物种：Elizabethkingia miricola

属性：Protein

标记：Unconjugated

产品描述（Product Details）

PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.

应用说明（Application）

Leaves N-glycan core oligosaccharides intact and suitable for further analysis.

Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination.

单位定义（Unit Definition）

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl.

纯度（Purity）

>90% as determined by SDS-PAGE.

酶活（Enzyme Activity）

>500 U/μL

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

注意事项（Notes）

To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.

PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with blue ice, please inquire the shipping cost.

存储（Storage）

This product is stable after storage at:

The product MUST be stored at -20°C or lower upon receipt.

-20°C for 12 months under sterile conditions.

PNGase F分子背景

PNGase F（肽 N- 糖苷酶 F）来源于 Elizabethkingia miricola，是一种高效的酰胺水解酶，可以裂解由天冬酰胺接的高甘露糖、杂合和复杂的寡糖糖蛋白。PNGase F 的切割位点为糖蛋白内侧 N- 乙酰葡萄糖胺（GlcNAc）和天冬酰氨残基之间的酰胺键，同时将酶解后蛋白上的天冬氨酰转化为天冬氨酸。