文章标题：The protective effects and mechanism of N-carbamoyl glutamate against H2O2-induced oxidative damage in IPEC-J2 cell
作者列表：Gao Mengmeng, Xie Zhijiang, Mao Pei, Zhang Xiaoli, Zhao Lingping, Ma Wenfeng
影响因子：2.6
期刊：BMC Veterinary Research
发表时间：2026-3-3
DOI:10.1186/s12917-025-05272-z
文献主题：Abstract
Background
This experiment aimed to explore the protective effects and mechanism of N-carbamoyl glutamate (NCG) against hydrogen peroxide (H2O2)-induced oxidative damage in intestinal porcine epithelial cells (IPEC-J2), providing a theoretical basis for the application of NCG in alleviating intestinal oxidative stress in weaned piglets. In this experiment, an oxidative stress model in IPEC-J2 cells were established using 400 µM H₂O₂, with an optimal NCG concentration (80 µM) determined via CCK-8 viability assay. Cells were divided into four groups: untreated group (Control), treated with 400 µM H2O2 group (H2O2), treated with 80 µM NCG group (NCG), and NCG pre-treatment (80 µM) followed by H2O2 (400 µM) group (NCG + H2O2).
