文章标题：Exploring the mechanism of Squamocin inhibits oral squamous cell carcinoma malignant progression by targeting peroxisome proliferator activated receptor-gamma based on network pharmacology and molecular docking
作者列表：Daoyong Hu, Qun Dai, Hao Xiong, Tian Zhong
影响因子：1.6
期刊：Letters in Drug Design & Discovery
发表时间：2026-3-7
DOI:10.1016/j.lddd.2025.100253
文献主题：Abstract
Background
Oral squamous cell carcinoma (OSCC) is a highly metastatic malignant tumor that originates from the squamous epithelium of oral mucosa. Squamocin, a compound derived from traditional Chinese medicine, has been shown to inhibit head and neck squamous cell carcinoma, yet its mechanism in OSCC remains unclear.
Methods
Cell functions were assessed using the 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU), flow cytometry, transwell, wound healing, and sphere-formation assays. Potential targets of Squamocin in OSCC were predicted via the SwissTargetPrediction and GeneCards databases. A protein-protein interaction (PPI) network was constructed with STRING and visualized in Cytoscape. Gene and protein expression levels were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot. Molecular docking and cell thermal shift assay (CETSA) were used to examine the binding between Squamocin and peroxisome proliferator activated receptor-gamma (PPARG).
