| Name | Z-FA-FMK |
| Description | Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases. |
| Cell Research | T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. (Only for Reference) |
| In vitro | Z-FA-FMK inhibits the development of Ras-induced carcinogenic tumors and respiratory viral infections in the cardiac tissue of mice with severe combined immunodeficiency (SCID). In a mouse model of intranasal pneumococcal infection, Z-FA-FMK significantly promotes the growth of Streptococcus pneumoniae in both the lungs and the bloodstream. |
| In vivo | Z-FA-FMK effectively inhibits T-cell proliferation induced by interleukin-2 (IL-2) and mitogens in vitro. It also prevents the degradation of fibrillar collagen through its actions on fibroblasts and osteoclasts. Furthermore, Z-FA-FMK suppresses the expression of NF-κB-dependent genes in macrophages, thereby inhibiting the production of cytokines induced by lipopolysaccharides. |
| Storage | store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.18 mM), Sonication is recommended.
DMSO : 71 mg/mL (183.74 mM), Sonication is recommended.
Ethanol : 32 mg/mL (82.81 mM), Sonication is recommended.
|
| Keywords | Z-FA-FMK | ZFAFMK | Z FA FMK | SARS-CoV | SARSCoV | SARS coronavirus | RRMs | reovirus replication | Inhibitor | inhibit | externalization of phosphatidylserine | DNA fragmentation | DEVDase | CysteineProtease | Cysteine Protease | Cysteine protease | Cathepsin | Caspase | Apoptosis |
| Inhibitors Related | Stavudine | Cysteamine hydrochloride | Sodium 4-phenylbutyrate | Hydroxychloroquine | Metronidazole | Citric Acid Triammonium | Tributyrin | L-Methionine | Cystamine dihydrochloride | L-Ascorbic acid sodium salt | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Highly Selective Inhibitor Library | Cysteine Covalent Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Anti-Viral Compound Library | Inhibitor Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Covalent Inhibitor Library | Fluorochemical Library | Anti-Infection Compound Library | Human Metabolite Library |
United States
