| Name | Thymidine |
| Description | Thymidine (DThyd) is a cell synchronizing agent and a specific precursor of deoxyribonucleic acid. Thymidine inhibits DNA synthesis and can cause cell cycle arrest in the G1/S phase. |
| In vitro | METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Thymidine (300-1200 µM) and H2O2 (1 mM) for 0.5 h. DNA damage was detected by comet assay.
RESULTS: For the control and Thymidine alone groups, the samples appeared almost circular due to the absence of DNA damage. After exposure to 1.0 mM H2O2 for 0.5 h, a clear comet was observed, indicating DNA damage in this group. However, there was a decreasing trend in the extent of DNA damage in the three combination groups as the concentration of Thymidine pretreatment increased. [1]
METHODS: Jurkat, MOLT4 and NALM6 cells were treated with Thymidine (2 mM) for 18 h.
RESULTS: Thymidine arrests the cell cycle at the G1/S boundary. [2] |
| In vivo | METHODS: To investigate the effects of folate analogs on superantigen-reactive peripheral T cells in vivo, Thymidine (500 mg/kg) was injected intraperitoneally into MTX- or tomudex-administered mice twice daily for ten days.
RESULTS: Both MTX and tomudex blocked the expansion and accelerated the peripheral clearance of Vβ8-selective T cells. Thymidine administration completely eliminated this effect, suggesting that inhibition of glycoside synthase (TS), but not other folate-dependent enzymes, is the predominant mechanism. [3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | Saline : 25 mg/mL (103.21 mM)
Ethanol : Soluble
DMSO : 50 mg/mL (206.42 mM)
Pyridine, Methanol, etc. : Soluble
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| Keywords | DNA/RNA Synthesis | Endogenous Metabolite | DNA | NSC21548 | arrest | synchronizing | replication | Inhibitor | NSC-21548 | Thymidine | precursor | inhibit |
| Inhibitors Related | Sucrose | Daidzein | Ferulic Acid | Glycerol |
| Related Compound Libraries | Marine Natural Product Library | Bioactive Compound Library | Selected Plant-Sourced Compound Library | EMA Approved Drug Library | Natural Product Library | Anti-infective Natural Product Library | Bioactive Compounds Library Max | Gut Microbial Metabolite Library |
United States
