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ChemicalBook Product Catalog Analytical Chemistry Analysis reagents General Analytical Reagents Ninhydrin

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Ninhydrin NEW

$ 29.00 /1g

Min. Order
Purity99.96%
Cas No485-47-2
Supply Ability10g
Update time2026-01-05

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	Ninhydrin
CAS No	485-47-2
EC-No
Min. Order
Purity	99.96%
Supply Ability	10g
Release date	2026/01/05

Product Introduction

Bioactivity

Name	Ninhydrin
Description	Ninhydrin is a chromogenic analytical probe for the quantification of proteins and amino acids.
In vitro	I. Amino Acid Analysis 1. Material Preparation 1) Ninhydrin solution: Prepared as 0.2%–1% ethanol or propanol solution. 2) Amino acid standard solution or sample to be tested. 3) Buffer: Commonly used citric acid buffer or other buffers with pH 5.5–6.5. 4) Heating equipment: such as water bath or constant temperature heating device. 2. Steps 1) Prepare the reaction system: Add a certain volume of sample solution and an appropriate amount of Ninhydrin solution (usually 1:1 of the sample volume) to the test tube. Add buffer to adjust the reaction conditions (optional). 2) Heating color development: Heat the reaction system at 90–100°C for 10–20 minutes to generate blue-purple or yellow compounds (Ruhemann's purple). 3) Cooling and detection: Cool the reaction system to room temperature; use a spectrophotometer to measure the absorbance at 570 nm (blue-purple) or other appropriate wavelengths to calculate the amino acid concentration. II. Protein detection 1.. Material preparation 1) Ninhydrin solution (0.5%–1% propanol solution). 2) Protein sample solution. 30 Buffer (such as phosphate buffer). 2. Steps 1) Reaction preparation: Add an equal volume of Ninhydrin solution to the sample solution and mix gently. 2) Heating color development: Heat the mixture to 90–100°C for 15 minutes. The color reaction depends on the type and concentration of amino acids. 3) Detection: After cooling, use a spectrophotometer to measure the absorbance at a specific wavelength (usually 570 nm) and use a standard curve for quantitative analysis. Precautions 1) Operation safety: Ninhydrin is a potential allergen. Gloves and goggles should be worn during operation, and experiments should be performed in a well-ventilated area. 2) Storage in the dark: Ninhydrin is light-sensitive. The solution should be stored in the dark. It is recommended to store it at 4°C for short-term storage and -20°C for long-term storage. 3) Optimize reaction conditions: According to the characteristics of the sample, appropriately adjust the pH, temperature and reaction time to obtain the best results.
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 240 mg/mL (1347.25 mM), Sonication is recommended. H2O : 20 mg/mL (112.27 mM), Sonication and heating to 60℃ are recommended.
Keywords	protein \| probe \| Ninhydrin \| Inhibitor \| inhibit \| Dye \| chromogenic analytical probe \| chromogenic \| amino
Related Compound Libraries	Bioactive Compound Library \| Cosmetic Ingredient Compound Library \| Bioactive Compounds Library Max \| Human Metabolite Library

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