Basic information Safety Related Supplier

ChemicalBook Product Catalog Analytical Chemistry Analysis reagents General Analytical Reagents Ninhydrin

1/1

Ninhydrin NEW

$ 29.00 /1g

Min. Order
Purity99.97%
Cas No485-47-2
Supply Ability10g
Update time2026-03-27

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

show more

Enterprise Verified

Business Bank account Basic Contact Infomation Business Address Trade Company

Chemical Properties

Product Name	Ninhydrin
CAS No	485-47-2
EC-No
Min. Order
Purity	99.97%
Supply Ability	10g
Release date	2026/03/27

Product Introduction

Bioactivity

Name	Ninhydrin
Description	Ninhydrin is a chromogenic analytical probe for the quantification of proteins and amino acids.
In vitro	I. Amino Acid Analysis 1. Material Preparation 1) Ninhydrin solution: Prepared as 0.2%–1% ethanol or propanol solution. 2) Amino acid standard solution or sample to be tested. 3) Buffer: Commonly used citric acid buffer or other buffers with pH 5.5–6.5. 4) Heating equipment: such as water bath or constant temperature heating device. 2. Steps 1) Prepare the reaction system: Add a certain volume of sample solution and an appropriate amount of Ninhydrin solution (usually 1:1 of the sample volume) to the test tube. Add buffer to adjust the reaction conditions (optional). 2) Heating color development: Heat the reaction system at 90–100°C for 10–20 minutes to generate blue-purple or yellow compounds (Ruhemann's purple). 3) Cooling and detection: Cool the reaction system to room temperature; use a spectrophotometer to measure the absorbance at 570 nm (blue-purple) or other appropriate wavelengths to calculate the amino acid concentration. II. Protein detection 1.. Material preparation 1) Ninhydrin solution (0.5%–1% propanol solution). 2) Protein sample solution. 30 Buffer (such as phosphate buffer). 2. Steps 1) Reaction preparation: Add an equal volume of Ninhydrin solution to the sample solution and mix gently. 2) Heating color development: Heat the mixture to 90–100°C for 15 minutes. The color reaction depends on the type and concentration of amino acids. 3) Detection: After cooling, use a spectrophotometer to measure the absorbance at a specific wavelength (usually 570 nm) and use a standard curve for quantitative analysis. Precautions 1) Operation safety: Ninhydrin is a potential allergen. Gloves and goggles should be worn during operation, and experiments should be performed in a well-ventilated area. 2) Storage in the dark: Ninhydrin is light-sensitive. The solution should be stored in the dark. It is recommended to store it at 4°C for short-term storage and -20°C for long-term storage. 3) Optimize reaction conditions: According to the characteristics of the sample, appropriately adjust the pH, temperature and reaction time to obtain the best results. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 240 mg/mL (1347.25 mM), Sonication is recommended. H2O : 20 mg/mL (112.27 mM), Sonication and heating to 60℃ are recommended.
Keywords	protein \| probe \| Ninhydrin \| Inhibitor \| inhibit \| Dye \| chromogenic analytical probe \| chromogenic \| amino
Related Compound Libraries	Bioactive Compound Library \| Cosmetic Ingredient Compound Library \| Bioactive Compounds Library Max \| Human Metabolite Library

show more

Company Profile Introduction

You may like

Recommended supplier

Ninhydrin hydrate

$10.00/1KG

Hebei Chuanghai Biotechnology Co., Ltd

VIP7Y

Ninhydrin hydrate

$0.00/25kg

Shaanxi Dideu New Materials Co. Ltd

VIP2Y

Ninhydrin hydrate

$9.00/1KG

Hebei Chuanghai Biotechnology Co,.LTD

VIP5Y

$30.00/1KG

Wuhan wingroup Pharmaceutical Co., Ltd

Ninhydrin hydrate

$15.00/1KG

Zhuozhou Wenxi import and Export Co., Ltd

$1.00/1Kg

Shaanxi Dideu Medichem Co. Ltd

VIP7Y

Ninhydrin hydrate

$8.00/1kg

Career Henan Chemical Co

VIP8Y

Ninhydrin hydrate

$15.00/1KG

Zhuozhou Wenxi import and Export Co., Ltd

Ninhydrin hydrate

$15.00/1KG

Zhuozhou Wenxi import and Export Co., Ltd

Send Inquriy