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485-47-2

Name Ninhydrin hydrate
CAS 485-47-2
EINECS(EC#) 207-618-1
Molecular Formula C9H6O4
MDL Number MFCD00003791
Molecular Weight 178.14
MOL File 485-47-2.mol

Chemical Properties

Description
Ninhydrin is historically the most common reagent for processing fingerprints on porous substrates. It has been used as a fingerprint reagent since the 1950s but has been used as a cell stain since the early 1900s. Ninhydrin molecules form a complex with amino acids resulting in a purple product known as Ruhmann's purple. Purple fingerprints are easily visible on light-colored porous substrates but may be difficult to see on patterned or dark-colored substrates. DFO reacts with amino acids to produce a fluorescent product that is not readily seen with the naked eye. This makes it a useful reagent on dark or patterned porous items. DFO prints are viewed with an alternate light source or laser set to 530–570 nm (green light) through a red barrier filter. 1,2-indanedione is an emerging fingerprint reagent that results in visible pink fingerprints that are also fluorescent. The fluorescence is viewed under the same conditions as DFO.
These chemicals can be used on their own or in sequence to develop latent prints on porous substrates. If they are used in sequence, they are used in the following order: indanedione, DFO, ninhydrin. If a reagent is used out of sequence, it will inhibit or limit the effectiveness of the other reagents. In this laboratory exercise you will develop fingerprints on a porous substrate using indanedione, DFO, and ninhydrin.
Appearance White to pale yellow crystalline powder
Melting point  250 °C (dec.) (lit.)
Boiling point  230.35°C (rough estimate)
density  0.862
vapor density  6.16 (vs air)
refractive index  1.4209 (estimate)
Fp  13 °C
storage temp.  Store at RT.
solubility  ethanol: soluble100mg/mL
form  Powder/Solid
pka 8.47±0.20(Predicted)
color  white to brownish-white, crystals
Specific Gravity 0.788
Odor Odorless
PH 4.6-5.0 (10g/l, H2O, 20℃)
PH Range 4.6 - 5.0
Stability: Stable. Incompatible with amines, alkalies, strong bases. Light-sensitive.
Water Solubility  0.1-0.5 g/100 mL at 20 ºC
Sensitive  Light Sensitive
Merck  14,6554
BRN  1910963
InChIKey FEMOMIGRRWSMCU-UHFFFAOYSA-N
LogP 0.670
Uses
Ninhydrin is an amino acid-sensitive reagent for the development of latent fingermarks on porous surfaces. More sensitive alternatives, such as DFO and IND-Zn, are now available and these have advantages on porous substrates that are not highly luminescent. However, ninhydrin can still be applied after these treatments and may reveal additional ridge detail, particularly on highly luminescent substrates where luminescence visualization may be problematic.
CAS DataBase Reference 485-47-2(CAS DataBase Reference)
NIST Chemistry Reference Ninhydrin(485-47-2)
EPA Substance Registry System 485-47-2(EPA Substance)

Safety Data

Hazard Codes  Xn,T,F
Risk Statements 
R22:Harmful if swallowed.
R36/37/38:Irritating to eyes, respiratory system and skin .
R39/23/24/25:Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed .
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed .
R11:Highly Flammable.
Safety Statements 
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) .
S16:Keep away from sources of ignition-No smoking .
S36:Wear suitable protective clothing .
RIDADR  UN 1170 3/PG 2
WGK Germany  3
RTECS  NK5425000
8
TSCA  Yes
HazardClass  3
PackingGroup  II
HS Code  29144000
Toxicity
LD50 orally in Rabbit: 600 mg/kg

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