| NADH (disodium salt) (606-68-8) |
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| NADH powder stands for “nicotinamide adenine dinucleotide (NAD) + hydrogen (H).” This chemical occurs naturally in the body and plays a role in the chemical process that generates energy. People use NADH supplements as medicine. |
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| Name | NADH(disodium salt) |
| CAS | 606-68-8 |
| EC Number: | 210-123-3 |
| Purity | 98% |
| Chemical name | beta-Nicotinamide adenine dinucleotide reduced disodium salt(NADH 2Na) |
| Synonyms | Coenzyme I reduced disodium salt, β-NADH, Diphosphopyridine nucleotide reduced disodium salt, β-DPNH |
| Molecular Formula | C21H27N7Na2O14P2 |
| Molecular Weight | 709.4 g/mol (anhydrous basis) |
| InChI Key | QRGNQKGQENGQSE-WUEGHLCSSA-L |
| Form | Solid |
| Appearance | White powder |
| Solubility | Soluble in water (100 g/l) at 20° C. |
| Storage Condition | Store in a sealed airtight container, keep the air out, protected from heat, light and humidity. |
| Use | Cosmetic，Dietary Supplement，IVD ，enzymatic synthesis（biocatalysis） |
| Testing Document | Available |
| Stability | Stable more than 6 months in room temperature |
| Particle size | 80 mesh |
| Storage Condition | Keep dry ,away from light, and store at 2℃- 8℃, long term store at -15℃ to -25℃ |
| Package | 10gram/bag ,50gram/bag , 1KG/bag, 25KG/drum |
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| DESCRIPTION |
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| Application |
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| Nicotinamide adenine dinucleotide (NADH) has been used in: |
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| nicotinamide adenine dinucleotide (NAD+) /NADH cycling assay. |
| adenosine triphosphate (ATP)ase assay. |
| pyruvate kinase enzyme assay. |
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| As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
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| Biochem/physiol Actions |
| NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. |
| As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
| Quality |
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| Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard) |
| Organic solvents: <4% (GC) |
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| Preparation Note |
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| Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C. |
| Analysis Note |
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| Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm |
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