| Name | Hygromycin B |
| Description | Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes. |
| In vitro | METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened.
RESULTS: Hygromycin B screened the lentivirally successfully transfected cells. [1]
METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter.
RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL. [2] |
| In vivo | METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days.
RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo. [3] |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 30 mg/mL (56.87 mM), Sonication is recommended.
5% DMSO+95% Saline : 1.58 mg/mL (3 mM), In vivo: Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
Saline : 50 mg/mL (94.78 mM), In vivo: Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
10% DMSO+90% Saline : 3 mg/mL (5.69 mM), In vivo: Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
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| Keywords | Inhibitor | inhibit | Hygromycin B | Fungal | Bacterial | Antibiotic | 70S ribosome |
| Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | Potassium gluconate | Ampicillin sodium | Methyl anthranilate | Kanamycin sulfate | Urethane | Sulfamethoxazole sodium | Doxycycline | Geraniol | Isoeugenol | Dimethyl sulfoxide |
| Related Compound Libraries | Anti-Parasitic Compound Library | Bioactive Compound Library | Anti-Fungal Compound Library | Natural Product Library | Saccharide and Glycoside Natural Product Library | Drug Repurposing Compound Library | Antiparasitic Natural Product Library | Microbial Natural Product Library | Natural Product Library for HTS | Immunology/Inflammation Compound Library | Anti-infective Natural Product Library | Bioactive Compounds Library Max |
United States
