| Name | Hygromycin B |
| Description | Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic cells by interfering with translocation and causing mistranslation by the 70S ribosome. It is commonly used for the selection of cells transfected with the hph or hyg resistance gene. |
| In vitro | METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened.
RESULTS: Hygromycin B screened the lentivirally successfully transfected cells. [1]
METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter.
RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL. [2] |
| In vivo | METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days.
RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo. [3] |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 240 mg/mL (454.96 mM), Sonication is recommended.
H2O : 125 mg/mL (236.96 mM), Sonication is recommended.
5% DMSO+95% Saline : 1.58 mg/mL (3 mM), Solution.
Saline : 50 mg/mL (94.78 mM), Solution.
10% DMSO+90% Saline : 3 mg/mL (5.69 mM), Solution.
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| Keywords | Inhibitor | inhibit | Hygromycin B | Fungal | Bacterial | Antibiotic | 70S ribosome |
| Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | Ampicillin sodium | Methyl anthranilate | Kanamycin sulfate | Sulfamethoxazole sodium | Terbinafine hydrochloride | Doxycycline | Penicillin G potassium | Geraniol | Dimethyl sulfoxide | Ethyl acetoacetate |
| Related Compound Libraries | Anti-Parasitic Compound Library | Bioactive Compound Library | Anti-Fungal Compound Library | Natural Product Library | Drug Repurposing Compound Library | Saccharide and Glycoside Natural Product Library | Antiparasitic Natural Product Library | Inhibitor Library | Microbial Natural Product Library | Natural Product Library for HTS | Anti-infective Natural Product Library | Bioactive Compounds Library Max |
United States
