DL-Dithiothreitol NEW

DL-Dithiothreitol has antioxidant effect, it can protect the reducing groups on the enzyme molecules, maintain the reducing environment, and stabilize the activity of the enzyme. DTT also contains some RNase inhibitors, which can inhibit the activity of RNase. It has a similar effect to mercaptoethanol (dimercaptoethanol), but DTT has a much less irritating odor and is much less toxic than mercaptoethanol. Moreover, when the concentration of DTT is 7 times lower than that of mercaptoethanol, the two effects are similar. But DTT prices are slightly higher. Cleavage and sequence analysis of protein disulfide bonds used as protein Euclidean protection agents. DL-Dithiothreitol is a small molecule organic reducing agent with the chemical formula C4H10O2S2, which is mainly used in the field of biotechnology as a protein cracking reagent. At present, the most widely used synthesis method of dithiothreitol is based on threitol as raw material, which is oxidized with potassium permanganate, then gets thiothreitol under the action of thioacetic acid, and finally gets dithiothreitol by hydrolysis of the thiointermediate.