| 名称 | ATP |
| 描述 | ATP (Adenosine triphosphate) provides cellular energy, participates in overall energy balance, and maintains intracellular homeostasis. ATP can act as an extracellular signaling molecule through interactions with specific purinergic receptors to mediate a variety of processes including neurotransmission, inflammation, apoptosis, and bone remodeling. |
| 体外活性 | METHODS: Synovial fibroblast HSF were rapidly treated (wash-in and wash-out ~2 s) with ATP (10 μM) three times, and [Ca2+] changes were detected using Fluo-4 fluorescence.
RESULTS: During the first application of ATP, Fluo-4 fluorescence increased rapidly after a delay of a few seconds and decreased slightly before the end of ATP application. After removal of ATP, the fluorescence signal returned to resting levels, but fluorescence decreased much more slowly than it had begun after the initial ATP application. The two subsequent ATP applications produced a response of less amplitude than the first, and the delay until the onset of the response appeared to lengthen with successive applications. [1]
METHODS: Mouse bone marrow-derived macrophages BMDM were stimulated with LPS, HKEC, or HKSA, followed by treatment with ATP (2 mM) for 0.5-24 h. Levels of IL-1β, KC, and MIP-2 were determined using ELISA.
RESULTS: ATP treatment strongly induced the secretion of IL-1β, KC and MIP-2. [2] |
| 体内活性 | METHODS: To test the antibacterial activity in vivo, ATP (50 mg/kg) was injected intraperitoneally into Kunming mice, and E. coli 25922 or S. aureus 25923 was injected 1-24 h later.
RESULTS: The administration of ATP 4 h or 24 h before the attack did significantly increase the survival rate of infected mice, regardless of the bacterial type. [2]
METHODS: To assay in vivo antibacterial activity, ATP (40 mg/kg), clarithromycin (12 mg/kg), and rifampin (8 mg/kg) were injected subcutaneously into MAC-infected BALB/c mice five times per week for eight weeks.
RESULTS: Co-administration of ATP with clarithromycin and rifampin accelerated bacterial clearance in MAC-infected mice without resulting in changes in histopathologic features or mRNA expression of pro- or anti-inflammatory cytokines in mice not given ATP. [3] |
| 存储条件 | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 1.67 mg/mL (3.29 mM)
H2O : 100 mg/mL (197.17 mM), Sonication is recommended.
|
| 关键字 | energy | Adenosine Triphosphate | inflammation | inhibit | Endogenous Metabolite | immunity | Inhibitor | inflammasome | Adenosine 5'-triphosphate | ATP disodium Hydrate | storage | Adenosine | cytokine | NLRP3 | metabolism | ATP |
| 相关产品 | Sucrose | Ferulic Acid | Glycerol | Thymidine |
| 相关库 | 经典已知活性库 | 中药单体化合物库 | 天然产物库 | 药物功能重定位化合物库 | 高通量筛选天然产物库 | 已知活性化合物库 | 肠道微生物代谢化合物库 |
United States
