Anti-NeuN (rabbit monoclonal), clone 27-4

Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of rat E18 cortex and hippocampus primary cultures using Cat. No. MABN140, Anti-NeuN (rabbit monoclonal), clone 27-4 and a Donkey Anti-Rabbit IgG secondary antibody conjugated to Cy3 (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.Western Blotting Analysis:
Representative lot data.
Mouse brain nuclear extract was probed with Cat. No. MABN140, Anti-NeuN (rabbit monoclonal), clone 27-4 (1:2,000 dilution).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates NeuN (~46 /48 kDa).

背景

NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

应用

抗NeuN（兔单克隆）抗体，克隆27-4检测NeuN（兔单克隆）的水平，并且已经发布并验证可用于ICC，Western Blotting和IHC。

简介

Anti-NeuN(rabbit monoclonal),clone 27-4是以Anti-NeuN为抗原以兔为宿主制得的NeuN(rabbit monoclonal)抗体，可以特异性结合兔NeuN。