Chlorophenol red-beta-D-galactopyranosid

Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.

Crystal

Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):

• in paper-based cell-free protein expression system for target RNA detection
• in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
• in colorimetric assay for bacteria quantification

Chlorophenol Red-β-D-galactopyranoside measures β-galactosidase expression levels in transfected cells. Chlorophenol Red-β-D-galactopyranoside is a substrate of β-Galactosidase.

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

[1] CLéMENCE SICARD. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-d-galactopyranoside[J]. Analytical and Bioanalytical Chemistry, 2014, 406 22: 5395-5403. DOI: 10.1007/s00216-014-7935-0
[2] CHARLOTTE JENDRESEN  Lars N G N  Michael R Daws. An improved CPRG colorimetric ligand-receptor signal transduction assay based on beta-galactosidase activity in mammalian BWZ-reporter cells[J]. Journal of pharmacological and toxicological methods, 2018, 90: Pages 67-75. DOI: 10.1016/j.vascn.2017.11.004