Take dry manipulation ivy stems and leaves 500g, crushed, with 30% ethanol 4000mL, 2000mL at 80 successively heated reflux for two times, reflux time were 3 hours, 2 hours. Combine the two extracts, decompression recovery of solvent, get ivy crude extract. The crude extract of ivy was dissolved in 150 mL of water in an ultrasonic generator, and the aqueous solution was extracted with equal volumes of ether, ethyl acetate and n-butanol for three times, respectively. The three n-butanol extracts were combined, concentrated under reduced pressure to near dryness and dissolved in 80 mL of 95% ethanol. Gradually add ethyl acetate to the 95% ethanol solution under stirring until no more precipitate was produced, and then collect the precipitate by filtration after 2 hours of standing. The precipitate was dissolved with a small amount of methanol and eluted by column chromatography using silica gel as stationary phase and a mixture of chloroform:methanol equal to 65:35 as mobile phase. The eluent was collected, and the collected eluent containing ivyoside C was concentrated and freeze-dried to obtain ivyoside C1.0759 g, and the content of ivyoside C was 98.2% as detected by high performance liquid chromatography.
