Hygrolidin
![Hygrolidin 结构式](https://img.chemicalbook.com/CAS/20180808/GIF/83329-73-1.gif)
Hygrolidin 性质
沸点 | 821.1±65.0 °C(Predicted) |
---|---|
密度 | 1.17±0.1 g/cm3(Predicted) |
储存条件 | Store at -20°C |
溶解度 | 溶于二甲基亚砜 |
形态 | 固体 |
酸度系数(pKa) | 3.28±0.10(Predicted) |
Hygrolidin 用途与合成方法
Hygrolidin (0.1, 1 μg/ml; for 24 h) increases both G1 and S phase populations and decreases M phase population.
Hygrolidin (0.1, 1, 10 μg/ml; for 24 h) decreases the amounts of cdk4, cyclin D, cyclin B and increases the amounts of cyclin E and p21. Hygrolidin-induced p21 preferentially associates with cyclin A-cdk2 complex and inhibits it.
Hygrolidin (0.1, 1 μg/ml; for 24 h) selectively induces p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts.
Hygrolidin, for 3 days, inhibits growth of various cell lines including DLD-1 colon cancer, LNCaP prostate cancer, K562 leukemia cells, LNCaP prostate cancer, EL-4 lymphoma (IC
50
s = 1.0-33 ng/ml, respectively).
Hygrolidin shows growth inhibition on
Trypanosoma cruzi
(IC
50
=1.1 nM),
Trypanosoma brucei brucei
(IC
50
=77 nM), and
Leishmania donovani
(IC
50
=72.5 nM).
Cell Cycle Analysis
Cell Line: | DLD-1 cells |
Concentration: | 0.1, 1 μg/ml |
Incubation Time: | For 24 hours |
Result: | Increased both G1 and S phase populations and decreased M phase population. |
Western Blot Analysis
Cell Line: | DLD-1 cells |
Concentration: | 0.1, 1, 10 μg/ml |
Incubation Time: | For 24 hours |
Result: | Decreased the amounts of cdk4, cyclin D, cyclin B and increased the amounts of cyclin E and p21. |
RT-PCR
Cell Line: | DLD-1 or WI-38 cells |
Concentration: | 0.1, 1 μg/ml |
Incubation Time: | For 24 hours |
Result: | Selectively induced p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts. |