Background
Homocysteine (Hcy) is an amino acid that requires vitamins B12 and folic acid for its metabolism. Vitamins B12 and folic acid deficiencies lead to hyperhomocysteinemia (HHcy, elevated Hcy), which is linked to the development of diabetic retinopathy (DR), age-related macular degeneration (AMD), and Alzheimer’s disease (AD).
Specific Modeling Methods
Male C57Mice: cbs+/-; 3-5 weeks C57BL6/J; 6-8 weeks of age
Administration: 1 μL intravitreally 200 μM
BL/6 mice (5 weeks old)
Note
(1) To avoid uncontrolled intraocular pressure increase, the volume of intravitreal injections is limited to 1 μL.
(2) L-Homocysteine thiolactone hydrochloride is dissolved in water and a working solution of 10x is prepared by diluting 1 μL of stock solution (200 mM) to 100 μL with PBS. Then by injecting 1 μL of this working solution, 200 μM vitreal concentration of Hcy-thiolactone is obtained.
Modeling Indicators
Molecular changes: Caused microglia activation in the retina. Increased indicators: pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6
Correlated Product(s): L-Homocysteine (HY-W010347)
Opposite Product(s): /
